Laboratory Methods for the Experimental Study of ImmunityF.J. Heer printing Company, 1909 - 197 sider |
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Vanlige uttrykk og setninger
24 hour culture 30 minutes agar agglutinating agglutinin alcohol amboceptor amount anaphylaxis animals antibodies antigen antitoxic bacteria bacterial suspension bactericidal Bactericidal Action Berkefeldt filter Bleed blood serum broth carbol-thionin Carefully cells centrifugate citrate cobra venom collect the serum complement Control Tube cytes cytotoxins defibrinated dilutions Diphtheria Antitoxin disease erythrocytes Exercise experiments fluid glanders goat erythrocytes graduated in 1/10 guinea pig guinea pig serum heated hemolysis hours at 37°C ice chest immunity unit injection inoculation instructor intraperitoneally L+ dose lecithin leucocytes loop Mallein method NaCl sol NaCl solution needle normal guinea pig note results oil immersion objective opsonic opsonic index opsonins phagocytic phagocytosis phenol pipette Place precipitins Proceed as follows Procure produce protein reaction rocytes sera Shake slide small test tubes Special Directions stain Standardization sterile glass student will proceed subcutaneously substances syphilis syringe temperature Tetanolysin Tetanospasmin Tetanus Antitoxin tetanus toxin tion tuberculin typhosis Vaccine virulent culture washed eryth
Populære avsnitt
Side 90 - The immunity unit for measuring the strength of tetanus antitoxin shall be ten times the least quantity of antitetanic serum necessary to save the life of a 350-gram guinea pig for ninety-six hours against the official test dose of a standard toxin furnished by the Hygienic Laboratory of the Public Health and Marine-Hospital Service.
Side 90 - ten times the least quantity of antitetanic serum necessary to save the life of a 350-gram guinea pig for ninety-six hours against the official test dose of a standard test toxin furnished by the Hygienic Laboratory of the Public Health and Marine Hospital Service.
Side 106 - To 500 cc of antitoxic serum add an equal volume of a saturated solution of ammonium sulphate and allow to stand 24 hrs.
Side 90 - ... against 20-gram mice, but against 350-gram guinea-pigs.) In the actual standardization of tetanus antitoxin, as in that of diphtheria antitoxin, the L+ dose of toxin is employed. The L+ dose is, however, in this case, defined as the smallest quantity of tetanus toxin that will neutralize one-tenth of an immunity unit, plus a quantity of toxin sufficient to kill a 350-gram guinea-pig in just four days. At the Hygienic Laboratory at Washington, a standard toxin and antitoxin are preserved under...
Side 3 - Ohio, 1909, pp. 196). — In this laboratory guide, the author takes up those general topics in connection with the phenomena of immunity with which the student of experimental medicine must be familiar before attempting to investigate any of the more important problems of immunopathology.
Side 34 - NaCl solution and incubating them for 48 hours at 37° C. At the end of this time filter through a Berkefeldt filter.
Side 26 - By the injection of bacteria of reduced virulence. 3. By the injection of dead bacteria. 4 By the injection of the secretory or excretory products of the bacteria.
Side 24 - Injections may be made in a variety of ways and in a variety of 'locations in the animal body.
Side 98 - ... necropsy done 48 hours after the subcutaneous injection of the mixture into a guinea pig.
Side 50 - The opsonic index is obtained by dividing the phagocytic index of the serum of a diseased animal with that of the serum of a normal animal, using the same leucocytic suspension.