5 Hæmatoxylin, Solution of. а. Prepare a saturated solution of crystallized calcic chloride in 70 per cent. alcohol; then add alum to saturation. b. C. Prepare a saturated solution of alum in 70 per cent. alcohol. Add 1 volume of a to 8 of b. To the mixture of a and b add a few drops of a saturated solution of pure hæmatoxylin in absolute alcohol. Filter. 6. Iodine, Solution of. Prepare a saturated solution of potassic iodide in distilled water; saturate this solution with iodine. Filter. Dilute to a brown sherry colour. 7. Magenta, Solution of. Dissolve I decigr. of crystallized magenta (roseine) in 160 cubic centimetres of distilled water: add 1 cub. cent. of absolute alcohol. Keep in a well-closed bottle. 8. Mayer's Solution. See note p. 8. 9. Müller's Solution. Bichromate of potash Sodic sulphate Distilled water 25 grammes. 10 grammes. 10. Osmic Acid, Solution of. Best bought ready made in the form of 1 per cent. solution. 11. Paraffin. Melt together one part of solid paraffin (paraffin candles will do), one part of paraffin oil and one part of pig's lard. A mixture in the above proportions gives, when it has cooled, a mass of the most generally useful consistency. To imbed an object, scoop a hole in a bit of the paraffin, place the object (the surface of which must be dry) in this hole and fill up the latter with some melted paraffin. 12. Pasteur's Solution. See note, p. 6. 13. Potash Solution. Dissolve 5 grammes of potassic hydrate in 100 cubic cent. of water. 14. Schultz's Solution. Dissolve some zinc in hydrochloric acid; permit the solution to evaporate, in contact with metallic zinc until it has attained a syrupy consistence. Saturate the syrup with potassic iodide, and then add enough iodine to make a dark sherry-coloured solution. The object to be stained must be placed in a little water, and then some of the above solution added. 15. Silver Nitrate, Solution of. Dissolve o'5 grammes of silver nitrate in 100 cubic cent. of distilled water. Keep in an opaque stoppered bottle. 16. Sodic Chloride, Solution of. (Normal saline solution. Salt solution.) Dissolve 75 grammes of sodic chloride in 1 litre of distilled water. INDEX. A. ABDUCENTES, nervi, 189 Alcoholic fermentation, 5, 9, 10 Alimentary canal, of Anodonta, Alternation of generations, 37, 47, Ambulatory limbs, 129, 151 Anodonta cygnæa, 107; Laboratory work, 113 Antennæ, 130, 153 Antennules, 130, 153 Anterior commissure, 186 Anterior abdominal vein, 180, Arachnoid membrane, 168 Arterial system, of Anodonta, 111 Artery, cœliac, 178, coeliaco-me- Ascospores, 8, 36, 41 Astacus fluviatilis, 127; Labora- Auditory organs, of Anodonta, 113, Cervical suture, 128, 140 Chara, 42; Laboratory work, 48 Chiasma, optic, 241 Chlorophyll, 11, 45, 49, 75, 100, 103 Choroid, coat, 247; plexus, 185 Clavicle, 222 Cloaca, of Anodonta, 109, 115; of Coeliac artery, 178 Coeliaco-mesenteric artery, 178, EAR, see Auditory organ Embryo, of Anodonta, 112, 126; Embryo, cell 61, 71, 86; sac, 71, Encephalon, 185, 239 Encystation, of Amoeba, 19; of Endopodite, 131, 142, 153 Endoskeleton, 169, 211 Endosperm, 71, 86 Endosporium, 36 Epicoracoid, 222 Epidermis, of Bean, 72, 79, 81; 261 179 Fenestra ovalis, 195, 217, 248 Fertilization, process of, in Ano- Foot, of Anodonta, 107, 115 102 Frog, 159; Laboratory work, 196 18 |