ium tularense antigen but positive with Brucella melitensis in dilutions up to 1 to 320.

On May 8, 1925, a second sample of blood was received which had been drawn the preceding day. The serum was absorbed with antigens of the abortus and the melitensus A varieties of Br. melitensis. The results, given in Table I, indicate that the infection was with the melitensis A variety. The blood clot was planted in broth and on glucose agar slopes, but Br. melitensis could not be cultivated.

TABLE I.-Absorption of agglutinins from the serum from the Rocky Mount,

N. C., patient indicate that the infection was with the melitensis A variety

Tested with antigen 456;1 serum

diluted i to,

Tested with antigen 428; serum

diluted i to

Treatment of serum

10 20 40 80 160 320 640 1,280 10 20 40 80 160 320 640 1, 280

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1 See Table I and III of the first paper of this bulletin for information concerning the strains used for these tests. Strain 456 is of the abortus variety, and strain 428 is of the melitensis A variety.

: 4, complete sedimentation; 3, supernatant turbidity as in a control tube containing 25 per cent as much antigen as in the tubes in which the test was carried out; 2, supernatant turbidity as in a control tube containing 50 per cent of the antigen; 1, supernatant turbidity as in a control tube containing 75 per cent of the antigen.

3 The absorptions were with antigens of a density of 75,000 parts per million in a 1 to 5 dilution of the Serum,

On May 16, 1925, Dr. Edward Francis, of the Hygienic Laboratory, saw the patient and obtained the following history:

The patient, D. C. F., about 35 years of age, conducts a store at Rocky Mount, N. C., where beef, pork, mutton, veal, chickens, and rabbits are sold. He denied having handled goat meat, except on one occasion two or three years ago, when he secured goat meat for some one who wanted it. He drinks a little raw dairy milk which is certified by the health department but is not pasteurized.

The patient grew up as a practical country butcher, slaughtering cattle and hogs, rarely sheep, but no goats. He has done no slaughtering for the past three years. Three or four years ago he had what the doctor called blood poisoning after sticking a bone in his thumb. A sore developed on his finger, he had fever, and was confined to bed for 30 days.

The present illness began about March 1, 1925. During March the patient managed to go about, but at nights he had chills, fever, and sweats. He was confined to bed during April, and lost 20 pounds in weight. He took 25 grains of quinine daily during April. He has been free of fever since May 5, but he has not regained his usual vigor,

Doctor Smith stated that no goat milk is sold in Rocky Mount, and to his knowledge there are no goat herds in the vicinity. There seems to be no possibility that this infection could have come from goats, but there is no indication from what other species of animal it was derived.


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