Supplement to the Journal of Venereal Disease Information, Utgave 22Federal Security Agency, U.S. Public Health Service, 1949 |
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0.85-percent saline 30 minutes Antigen dilution antigen emulsion antigen particles antigen suspension bottle cardiolipin cardiolipin antigen cell suspension centrifuge centrifuge tube cholesterol cholesterolized alcohol clumps complement serum complement-fixation complement-fixation tests control tube decanting discard distilled water ether flask Flocculation Test glass glass-stoppered glycerinated guinea pig guinea pig serum heated serum hemolysin titration highest dilution Kahn antigen Kahn shaking machine Kahn test Kolmer lecithin merthiolate microscopically mix contents Mix tube negative serum paraffin percent Pipette Pipette 0.5 ml positive reaction positive serum pretested Quantitative Tests rack by hand rack of tubes reactivity Reading and Reporting refrigerator room temperature Rotate saline controls sensitized antigen suspension serologic tests serum dilution serum or spinal serum tests Shake rack slide sodium chloride sodium chloride solution spinal fluid tests stopper syphilis test tubes Test with Serum Test With Spinal tests for syphilis titer Transfer 0.5 ml Tube 1 Tube VDRL volume water bath
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Side 57 - Empty the salt solution into the' antigen and as rapidly as possible (without waiting to drain the tube) pour the mixture back and forth 12 times. Permit the mixture to stand for 30 minutes instead of the usual 10-minute period.
Side 110 - Add 4.1 ml. buffered saline from 5.0 ml. pipette. 6. Place top on bottle and shake vigorously for approximately 10 seconds. 7. Antigen emulsion is then ready for use and may be used during 1 day. This amount (5.0 ml.) is sufficient for approximately 250 serum tests. Twice this amount of antigen emulsion may be prepared at one time in a 30-ml.
Side 69 - Co.) should also be set at 180 rpm The Fisher-Kline rotator has a greater speed and smaller amplitude. When tests are performed in a hot, dry climate, slides may be covered with a box lid containing a moistened blotter, during rotation, to prevent excess evaporation. 4. Examine the reactions microscopically, employing a 100 X magnification. 5. Report observed results in accordance with the following outline : a. Typical reactions: Negative Antigen panicles dispersed, no clumping.
Side 110 - Add 0.5 ml of antigen (from the lower half of a 1.0-ml pipet graduated to the tip) directly onto the saline while continuously but gently rotating the bottle on a flat surface. Note: Antigen is added drop by drop, but rapidly, so that approximately 6 seconds are allowed for each 0.5 ml of antigen.
Side 79 - After these reagents are prepared the complement and hemolysin titrations may be assembled. Complement and Hemolysin Titrations 1. Perform these two titrations simultaneously in the same rack. 2. Place 10 tubes (numbered 1 to 10) in one side of the rack for the hemolysin titration and 8 tubes (numbered 1 to 8) in the other side for the complement titration. Add two other tubes to the rack, one for 1:1,000 hemolysin solution and one for 1:30 complement dilution.
Side 116 - Spinal fluids which are visibly ~m . contaminated or contain gross blood are unsatisfactory for testing. 2. Heat spinal fluid at 56° C. for 15 minutes. Cool to room temperature before testing. Preparation of the Sensitized Antigen Emulsion 1. Prepare antigen emulsion as described for the VDRL slide flocculation tests (see "Preparation of Antigen Emulsion
Side 36 - Few small clumps at the meniscus of either tube. In such instances, clumps should be redispersed by thumping the tube with a finger and the tube recentrifuged for 3 minutes. The test is reported doubtful...
Side 7 - ... Preparation of Serums 1. Remove serums from clots by centrifuging and pipetting or decanting. 2. Heat the serums in the 56° C. water bath for 30 minutes. Serums should not be heated before the day of testing. If it is necessary to retest a specimen, use serum freshly separated from the clot, if available. 3. Recentrifuge any specimen in which visible particles have formed during heating. Preparation of Glycerinated Hinton Indicator 1. Pipette one part of Hinton stock indicator into one compartment...
Side 75 - Add one drop of washed, packed, sheep red cells to each serum and mix well. c. Return all tubes to the refrigerator for 15 minutes. d. All tubes then are centrifuged and the serums separated by e.